Lstc Mac Process Manual
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During my second Master internship, under the guidance of Dr. Marc Baaden, Dr. Serge Perez and Dr. Anne Imberty.This internship project aimed to develop new kind of standardized visualizations for complex carbohydrate molecules.
Background
In Nature, carbohydrates form an important family of biomolecules. Carbohydrates, in the form of polysaccharides, glycopeptides, glycolipids, glycosaminoglycans, proteoglycans, or other glycoconjugates have long been recognize to participate in many biological processes. They can be present in very diverse and complex forms. In a similar fashion to proteins with amino acids, we can build complex carbohydrates from individual units: monosaccharides. But whereas the proteic alphabet is made of “only” 20 letters, around 120 monosaccharides are known and the fact that they can be linked through different positions adds even more complexity to the whole construction process.
Methods
Those who have a support contract with LST can contact us directly by email or phone. Others should contact their local distributor or use the resources listed below. LS-OPT Overview README.txt; LS-OPT 5.2 User Manual (Dec 2015) LS-OPT 4.2 User Manual (May 2011) LS-OPT 4.1 User Manual (December 2010) LS-OPT 4.0 User Manual (April 2009) LS-TaSC Manual. LS-TaSC 4.2 User Manual (October 2020) LS-TaSC 2.0 User Manual (April 2011) LSTC License Manager Software. The resources provided below describe LSTC’s academic programs and institutional policies and procedures. All information is subject to change without notice or obligation. Academic Catalog. 2020-2021 LSTC Catalog; 2019-2020 LSTC Catalog; 2018-2019 LSTC Catalog; 2016-2018 LSTC Catalog; All Students Handbook. 2020-2021 All Students Handbook.
Using the molecular visualization software UnityMol[1] engine it is easy to represent proteins, RNA/DNA and biological networks. UnityMol is based on a new molecular representation called HyperBalls[2] properties to draw very large complexes of several thousand atoms without screen lag. based on the Unity3D video game that uses graphical and shader properties to draw very large complexes of several thousand atoms without screen lag.
This software is developed within Marc Baaden’s team and is currently updated by Marc’s PhD student : Sebastien Doutreline. A lot of great features and performance improvements to come!
The software is available on the SourceForge repo : http://sourceforge.net/projects/unitymol/. UnityMol is also multi platform (Linux, Mac & Windows)
Feel free to download the tutorial too if you feel lost (it’s still a development interface).
Results
Fig 1 – Pentasaccharide LSTc (Glc,Gal,GlcNAc, Gal, NeuAc). Ligand of the Polyomavirus capsid protein (3NXD)
The first objectif was to integrate a color code for each monosaccharide. Serge Perez developed a colored pictogram code for every monosaccharide (http://glycopedia.eu/120-Monosaccharides). We wanted to integrate this color code to UnityMol. On the Fig 1 you can see an example of this color code with the pentasaccharide LSTc. You can find the correspondence of every color inside the Tutorial for SweetUnityMol.
Pentasaccharide LSTc in Licorice representation and RingBlending mode enabled.
The second representation, RingBlending, aims to fill aromatic rings of monosaccharide. this feature is inspired by a feature in the software VMD called « PaperChain » [3] (Cf. Fig 2). The monosaccharide color code is also implemented.
A third representation, called SugarRibbons, aims to reproduce the secondary structure of a carbohydrate molecule. Like the « cartoon » representation for proteins, SugarRibbons will follow the backbone of the polysaccharide and will be thicker arround the aromatic ring (Cf. Fig 3). The monosaccharide color code is also implemented and you can represent the position of the oxygen by a colored sphere (to have a better view of the monosaccharide conformation). This representation fit the atoms position, so despite the approximation of the visualization of the secondary structure, the SugarRibbons visualization stay very close to the polysaccharide structure and we can still be able to see atoms fluctuation between 2 structures.
Pentasaccharide LSTc in SugarRibbons mode and oxygen atoms represented by red spheres.
More images and examples can be seen on my Gallery page.
Ressources
Lstc Mac Process Manual 2020
- You can find more information on the Glycopedia website, maintened by Serge Perez : http://glycopedia.eu/Presentation-200.html.
- Or on our article :10.1093/glycob/cwu133
Lstc Mac Process Manual 2019
References
[1] Chavent, M., Vanel, A., Tek, A., Levy, B., Robert, S., Raffin, B., & Baaden, M. (2011). GPU-accelerated atom and dynamic bond visualization using hyperballs: a unified algorithm for balls, sticks, and hyperboloids. Journal of Computational Chemistry, 32(13), 2924–35. doi:10.1002/jcc.21861
[2] Lv, Z., Tek, A., Da Silva, F., Empereur-mot, C., Chavent, M., & Baaden, M. (2013). Game on, science – how video game technology may help biologists tackle visualization challenges. PloS One, 8(3), e57990. doi:10.1371/journal.pone.0057990
[3] Cross, S., Kuttel, M. M., Stone, J. E., & Gain, J. E. (2009). Visualisation of cyclic and multi-branched molecules with VMD. Journal of Molecular Graphics & Modelling, 28(2), 131–9. doi:10.1016/j.jmgm.2009.04.010
Lstc Mac Process Manual Pdf
Contributors : Marc Baaden, Matthieu Chavent, Alex Tek, Zhihan Lv, Franck Da Silva, Charly Empereur-mot, Sebastien Doutreline, Mikael Trellet, Caroline Roudier.